In vitro Exposure of Rabbit Ova to Estrogens.

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Abstract
Summary One-day ova of the rabbit were removed from the reproductive tract of the mother, exposed in vitro to certain sex steroids, and then returned to the reproductive tract of a pseudopregnant rabbit for completion of their development. Ova incubated for 5 minutes at room temperature in 0.1 mg/ml estradiol, 0.01 mg/ml estradiol, 0.1 mg/ml stilbesterol or 0.01 mg/ml stilbesterol survived as well as control ova incubated without steroid, and no abnormalities of the reproductive tract were observed. When the incubation period with the steroid was extended to 24 hours at a temperature of 10°C, 1 mg/ml estradiol, 0.1 mg/ml estradiol, and 1 mg/ml stilbesterol were lethal to the ova. Of 404 ova treated with 0.1 mg/ml stilbesterol for 24 hours at 10°C, 24 young rabbits were recovered. These included 17 normal females, 4 normal males, and 3 animals whose gonads consisted of testicular tissue without normal tubule formation, and whose gonads were in an intermediate anatomical position between that of normal males and females.

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