Minimal Residual Disease after Bone Marrow Transplant for Chronic Myeloid Leukaemia Detected by the Polymerase Chain Reaction
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Leukemia & Lymphoma
- Vol. 11 (sup1) , 39-43
- https://doi.org/10.3109/10428199309047861
Abstract
We describe the methodology and application of the polymerase chain reaction to detect BCR-ABL mRNA as a marker for CML cells. The technique is highly sensitive enabling the routine detection of 1 leukaemic cell in 105 or 106 normal cells and is therefore the most sensitive method available for detecting minimal residual disease. Analysis of marrow or blood from 80 patients after bone marrow transplantation for CML shows that residual leukaemia is often detectable for several months but that most subsequently become PCR negative. Patients who relapsed were all PCR positive before the detection of Philadelphia positive metaphases in bone marrow aspirates.Keywords
This publication has 6 references indexed in Scilit:
- Prognostic significance of Philadelphia chromosome-positive cells detected by the polymerase chain reaction after allogeneic bone marrow transplant for chronic myelogenous leukemiaBlood, 1992
- Clinical significance of bcr-abl gene rearrangement detected by polymerase chain reaction after allogeneic bone marrow transplantation in chronic myelogenous leukemiaBlood, 1991
- Detection of residual leukemia after bone marrow transplant for chronic myeloid leukemia: role of polymerase chain reaction in predicting relapseBlood, 1991
- False-positive results with PCR to detect leukaemia-specific transcriptThe Lancet, 1990
- Avoiding false positives with PCRNature, 1989
- Bone Marrow Transplantation for Chronic Myelogenous Leukemia in Chronic PhaseAnnals of Internal Medicine, 1988