Characterization of the NHA1 gene encoding a Na+/H+‐antiporter of the yeast Saccharomyces cerevisiae
- 27 May 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 387 (1) , 89-93
- https://doi.org/10.1016/0014-5793(96)00470-x
Abstract
The NHA1 gene (2958 nt) encoding a putative +/ + ntiporter (986 aa) in Saccharomyces cerevisiae was cloned by selection based on increased NaCl tolerance. The putative protein is highly similar to sodium/proton antiporters from Schizosaccharomyces pombe (gene sod2), and Zygosaccharomyces rouxii (gene Z-SOD2). Overexpression of the NHA1 gene results in higher and partially pH-dependent tolerance to sodium and lithium; its disruption leads to an increased sensitivity towards these ions.
Keywords
This publication has 20 references indexed in Scilit:
- The Yeast YBR235W Gene Encodes a Homolog of the Mammalian Electroneutral Na+-(K+)-Cl− Cotransporter FamilyBiochemical and Biophysical Research Communications, 1995
- BAP2, a gene encoding a permease for branched-chain amino acids in Saccharomyces cerevisiaeBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1995
- Three aspartic residues in membrane-spanning regions of Na+/H+ antiporter from Vibrio alginolyticus play a role in the activity of the carrierBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1995
- Functional expression of the ENA1(PMR2) - ATPase of Saccharomyces cerevisiae in Schizosaccharomyces pombeBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1995
- Na+-ATPases and Na+/H+ antiporters in fungiBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1994
- Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1993
- Determination of a specific region of the purine–cytosine permease involved in the recognition of its substratesMolecular Microbiology, 1992
- A novel P‐type ATPase from yeast involved in sodium transportFEBS Letters, 1991
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeastsGene, 1980