Signature-Tagged Transposon Mutagenesis Identifies Novel Mycobacterium tuberculosis Genes Involved in the Parasitism of Human Macrophages
Open Access
- 1 January 2007
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 75 (1) , 504-507
- https://doi.org/10.1128/iai.00058-06
Abstract
Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.Keywords
This publication has 31 references indexed in Scilit:
- Correlating sequential homology of Mce1A, Mce2A, Mce3A and Mce4A with their possible functions in mammalian cell entry of Mycobacterium tuberculosis performing homology modelingTuberculosis, 2005
- Genetic requirements for mycobacterial survival during infectionProceedings of the National Academy of Sciences, 2003
- Transcriptional Adaptation of Mycobacterium tuberculosis within MacrophagesThe Journal of Experimental Medicine, 2003
- Constrained Intracellular Survival ofMycobacterium tuberculosisin Human Dendritic CellsThe Journal of Immunology, 2003
- Mycobacterium tuberculosis Genes Induced during Infection of Human MacrophagesInfection and Immunity, 2002
- How can immunology contribute to the control of tuberculosis?Nature Reviews Immunology, 2001
- Identification ofMycobacterium tuberculosisRNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS)Proceedings of the National Academy of Sciences, 1999
- Survival of mice infected with Mycobacterium smegmatis containing large DNA fragments from Mycobacterium tuberculosisTubercle and Lung Disease, 1999
- Search for Genes Potentially Involved inMycobacterium tuberculosisVirulence by mRNA Differential DisplayBiochemical and Biophysical Research Communications, 1999
- Attenuation of Virulence by Disruption of the Mycobacterium tuberculosis erp GeneScience, 1998