Poly(A) tail length of oxytocin- and lysine vasopressin-encoding mRNAs increases during development in the porcine hypothalamus
- 1 April 1990
- journal article
- research article
- Published by Bioscientifica in Journal of Molecular Endocrinology
- Vol. 4 (2) , 151-158
- https://doi.org/10.1677/jme.0.0040151
Abstract
Lysine vasopressin- and oxytocin-encoding mRNAs have been analysed in the developing hypothalamus of the pig. The two hormone-encoding mRNAs were first detectable on fetal day 49 by Northern blot analysis. Whereas RNase mapping revealed identical transcripts throughout the developmental stages studied, Northern blots showed that the early transcripts appeared to be shorter (by 100–200 nucleotides) and more heterogeneous in size than those of later stages. This developmentally related length polymorphism was shown to be due to different poly(A) lengths and was abolished by removal of the poly(A) tails with RNase H. These results indicate that maturation of neurones in the developing porcine hypothalamus is accompanied by an increase in length of the poly(A) tail of vasopressin and oxytocin mRNAs.This publication has 16 references indexed in Scilit:
- The vasopressin mRNA poly(A) tract is unusually long and increases during stimulation of vasopressin gene expression in vivo.Molecular and Cellular Biology, 1988
- cDNA cloning of the immunoglobulin heavy chain binding protein.Proceedings of the National Academy of Sciences, 1988
- Expression of a mutant vasopressin gene: differential polyadenylation and read-through of the mRNA 3′ end in a frame-shift mutant.The EMBO Journal, 1986
- The Neurohypophyseal Hormones Vasopressin and Oxytocin. Precursor Structure, Synthesis and RegulationBiological Chemistry Hoppe-Seyler, 1986
- The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis.The EMBO Journal, 1984
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Identification of two distinct regulatory regions adjacent to the human β-interferon geneCell, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.Proceedings of the National Academy of Sciences, 1977