His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors
- 1 January 2004
- journal article
- research article
- Published by Springer Nature in Journal of Structural and Functional Genomics
- Vol. 5 (3) , 217-229
- https://doi.org/10.1023/b:jsfg.0000031965.37625.0e
Abstract
We have compared four different vectors for expression of proteins with N- or C-terminal hexahistidine (His6) tags in Escherichia coli by testing these on 20 human proteins. We looked at total...Keywords
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