Basement membrane collagen ‐ evidence for a novel molecular packing

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Abstract
Type IV collagen is the major structural protein of basement membranes but very little is known about its molecular organisation in vivo. We have used X‐ray diffraction of a thick basement membrane, bovine lens capsule, to provide information. Under constant load, lens capsule gave a collagen diffraction pattern of a similar quality to unstretched rat rail tendon. In addition there were clear meridional reflections which indexed as orders of 10 nm, and equatorial reflections at 2.1 and 3.8 nm. These results suggest the ordering of type IV collagen molecules in fibrils, with a 10 nm periodicity along the length of the fibrils.