Comparison Between Silica-based Methods for the Extraction of DNA from Human Bones from 18th to Mid-19th Century London
- 1 January 2002
- journal article
- research article
- Published by Taylor & Francis in Ancient Biomolecules
- Vol. 4 (4) , 173-178
- https://doi.org/10.1080/1358612021000028470
Abstract
We compared five silica-based methods for their efficiency at extracting DNA from human bones from 18th to mid-19th century London. Polymerase chain reactions (PCRs) were directed at two mitochondrial and one nuclear loci. Methods that used commercially available silica columns were more efficient than methods that used a silica slurry. Extraction with Qiagen QIAquick columns and the Qiagen QIAamp DNA Mini Kit were equally efficient, though we recommend the former as this method involves fewer steps.Keywords
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