Effect of potassium on ammoniagenesis by renal mitochondria
- 1 July 1976
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Legacy Content
- Vol. 231 (1) , 44-51
- https://doi.org/10.1152/ajplegacy.1976.231.1.44
Abstract
Ammonia production by rat renal cortical mitochondria was studied with both in vivo and in vitro manipulation of potassium to further elucidate the mechanisms relating potassium homeostasis and renal ammonia production. Mitochondria from potassium-depleted animals demonstrated an increase in ammonia production at all glutamine concentrations studied, which ranged from 0.5 to 10 mM. This increase in ammoniagenesis compared favorably in degree of change with and was of sufficient absolute magnitude to entirely account for the findings observed both in renal cortical slices studied in vitro and in the intact animal. When rotenone is added to the medium, increased ammoniagenesis is still detected, indicating that either glutamine entry into the mitochondria and/or the activity of phosphate-dependent glutaminase are critical rate-controlling steps. In contrast to studies with renal slices, a decrease in ammonia production was not apparent with cortical mitochondria from chronically potassium-loaded animals. In vitro alterations of the potassium homeostasis. Therefore, these experimental manipulations either do not activate an effector mechanism which takes place in vivo, or the alteration in ammoniagenesis requires an adaptation over time that is not achieved with this in vitro approach.This publication has 14 references indexed in Scilit:
- [15] Isolation of liver or kidney mitochondriaPublished by Elsevier ,1967
- Experimental Metabolic Acidosis: The Enzymatic Basis of Ammonia Production by the Dog Kidney*Journal of Clinical Investigation, 1965
- Renal ammonia release during ammonium chloride acidosisAmerican Journal of Physiology-Legacy Content, 1964
- AMINO ACID EXTRACTION AND AMMONIA METABOLISM BY THE HUMAN KIDNEY DURING THE PROLONGED ADMINISTRATION OF AMMONIUM CHLORIDE*Journal of Clinical Investigation, 1963
- Extraction of amino acids from and their addition to renal blood plasmaAmerican Journal of Physiology-Legacy Content, 1963
- Relation of renal amino and amide nitrogen extraction to ammonia productionAmerican Journal of Physiology-Legacy Content, 1963
- Exchange and diffusion of potassium in kidney cortex slicesAmerican Journal of Physiology-Legacy Content, 1962
- THE EFFECT OF THE ADMINISTRATION OF SODIUM BICARBONATE AND AMMONIUM CHLORIDE ON THE EXCRETION AND PRODUCTION OF AMMONIA. THE ABSENCE OF ALTERATIONS IN THE ACTIVITY OF RENAL AMMONIA-PRODUCING ENZYMES IN THE DOGJournal of Clinical Investigation, 1959
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956
- Kidney Glutaminase and Carbonic Anhydrase Activity and Tissue Electrolyte Composition in Potassium-Deficient DogsAmerican Journal of Physiology-Legacy Content, 1955