Identification of the sigB Operon in Staphylococcus epidermidis : Construction and Characterization of a sigB Deletion Mutant
Open Access
- 1 December 2001
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (12) , 7933-7936
- https://doi.org/10.1128/iai.69.12.7933-7936.2001
Abstract
The role of the alternative sigma factor ςB inStaphylococcus epidermidis was investigated by the construction, complementation, and characterization of asigB deletion mutant. Electrophoretic analyses confirmed a profound influence of ςB on the expression of exoproteins and cytoplasmic proteins. Detailed investigation revealed reduced lipase and enhanced protease activity in the ςB mutant. Furthermore, no significant influence of ςB on heterologous biofilm formation or on the activity of the global regulator agr was detected.This publication has 36 references indexed in Scilit:
- Alternative Transcription Factor ς B Is Involved in Regulation of Biofilm Expression in a Staphylococcus aureus Mucosal IsolateJournal of Bacteriology, 2000
- Construction and Characterization of an agr Deletion Mutant of Staphylococcus epidermidisInfection and Immunity, 2000
- Gene replacement in Staphylococcus carnosus and Staphylococcus xylosusFEMS Microbiology Letters, 1997
- Gene replacement in Staphylococcus carnosus and Staphylococcus xylosusFEMS Microbiology Letters, 1997
- Molecular basis of intercellular adhesion in the biofilm‐forming Staphylococcus epidermidisMolecular Microbiology, 1996
- Inducible production and cellular location of the epidermin biosynthetic enzyme EpiB using an improved staphylococcal expression systemFEMS Microbiology Letters, 1996
- A promoter-screening plasmid and xylose-inducible, glucose-repressible expression vectors for Staphylococcus carnosusGene, 1995
- Transformation ofStaphylococcus epidermidisand other staphylococcal species with plasmid DNA by electroporationFEMS Microbiology Letters, 1990
- Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporationFEMS Microbiology Letters, 1990
- Antibiotic penetration through bacterial capsules and exopolysaccharidesJournal of Antimicrobial Chemotherapy, 1982