Light Scattering Studies on α-Crystallin from Bovine Eye Lens
- 1 January 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 87 (1) , 15-21
- https://doi.org/10.1093/oxfordjournals.jbchem.a132720
Abstract
Using the usual angular light scattering technique, we have measured the concentration dependence of reduced scattering intensity of fractionated bovine lens α-crystallin (αA-chain) in the presence of 30 mM calcium chloride and 0.1 M sodium chloride. The inverse intensity of scattered light at zero angle per unit concentration of α-crystallin has a significant upward curvature with increasing α-crystallin concentration. The third virial coefficient determined from this upward curvature was 1.24×10−3 (mol1/2 ml1/2 g−1).Keywords
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