Radioisotopic quantitation in microtitration plates by an autofluorographic method
- 1 July 1978
- journal article
- Published by Wiley in Cell Biology International Reports
- Vol. 2 (4) , 375-380
- https://doi.org/10.1016/0309-1651(78)90023-1
Abstract
An autofluorographic record of drug titrations in microtitration plates has been obtained using cultures labelled with [35s] methionine after treatment with cytostatic drugs. By adding scintillation fluid directly to each culture well of the microtitration plate, and then centrifuging the evaporate the toluene and leave a flat even film of fluor, in situ scintillation is produced. When X-ray film is exposed to the plate the scintillation of individual wells is recorded as spots on the film. These may be interpreted by eye or by scanning densitometry.Keywords
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