Neuropeptide FF reverses the effect of μ-opioid on Ca2+ channels in rat spinal ganglion neurones

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Abstract
SINGLE neurones isolated from spinal ganglia of young rats were loaded with Fluo3, a fluorescent indicator of intracellular Ca2+ concentration. In 27 of 47 neurones the depolarization-evoked rise in fluorescence was reduced by 47.8 ± 3.3% by prior perfusion with DAGO (1 μM, 30s) a μ-opioid agonist. In 12 neurones an analogue of neuropeptide FF ((1DMe)Y8Fa, 10 nM, 30 min) did not affect the Ca2+ response to depolarization. (1DMe)Y8Fa reversed the effect of DAGO by 63 ± 8% in seven of these 12 neurones. We conclude that stimulation of neuropeptide FF receptors antagonizes μ-opioid modulation of Ca2+ channels at the cellular level.

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