Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis
- 1 May 1992
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (9) , 2742-2747
- https://doi.org/10.1128/jb.174.9.2742-2747.1992
Abstract
We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC[GGCCGC), SgrAI (C(A/G)[CCGG(T/G)G), and Sse8387I (CCTGCA[GG), and we analyzed the fragments by pulsed-field gel electrophoresis. A total of 25 restriction endonuclease sites and 13 genes and/or operons were located on the map. The genome size was determined to be 1,045 kb. Neither highly transcribed chlamydia genes nor developmental cycle-specific genes were clustered on the genome.Keywords
This publication has 41 references indexed in Scilit:
- Chlamydia trachomatis contains a protein similar to the Legionella pneumophila mip gene productMolecular Microbiology, 1991
- Cysteine‐rich outer membrane proteins of Chlamydia trachomatis display compensatory sequence changes between biovariantsMolecular Microbiology, 1990
- Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatisGene, 1988
- Developmental Regulation of the Cysteine-rich Outer-membrane Proteins of Murine Chlamydia trachomatisMicrobiology, 1988
- Restriction endonucleases for pulsed field mapping of bacterial genomesNucleic Acids Research, 1987
- A common plasmid of Chlamydia trachomatisPlasmid, 1986
- Expression of the Chlamydial Genus-Specific Lipopolysaccharide Epitope in Escherichia coliScience, 1985
- Cloning and expression inEscherichia coliof a species-specificChlamydia trachomatisouter membrane antigenFEMS Microbiology Letters, 1985
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Trachoma agent DNAJournal of Molecular Biology, 1969