THE ISOLATION AND PARTIAL CHARACTERIZATION OF THE GLYCOLIPIDS OF BP8/C3H ASCITES-SARCOMA CELLS

Publisher Website
Google Scholar
Abstract
The total lipid was extracted from BP8/C3H ascites-sarcoma cells with acetone, light petroleum, pyridine and chloroform-methanol successively. Each extract was treated with alkali. The alkali-stable lipids from the pyridine and chloroform-methanol extraces, which included the glycolipids, were fractionated on silicic acid and silica gel G col-ums. The total yield of glycolipid was about 60 mg/100 g dry wt of tumor cells, about 0.4% of the total lipid. Four classes of glycolipid were isolated and characterized as ceramide monohexoside (Gl), cera-nide dihexoside (G2), ceramide trihexoside (G3), and ceramide hexo-saminyltrihexoside (G4). Gl, G2, G3 and G4 constituted 55, 21, 9 and 15% of the total glycolipid respectively. Gl was a nixture of ceramide glucoside (70%) and ceramide galactoside. The general structures of the oligsaccharide moieties of G2, G3 and G4 were elucidated by partial acid hydrolysis of the glycolipids with water-soluble polystyrenesul-phonic acid. G2 was nostly ceramic lactoside with about 10% of ceramide galactosylgalactoside. G3 and G4 were probably a ceramide diga-lactosylglucoside and a ceramide N-acetyldalactosaminylgalactosylgalac-tosylglucoside respectively. The fatty acid compositions of the glycolipids were very similar; lingnoceric acid and nervonic acid were the major components and all contained monohydroxy acids in proportions varying from 10-25% of the total acids.