The quantitative determination of barbiturates in tissues by ultraviolet absorption spectrophotometry

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Abstract
A procedure based on u-v. spectrophotometry is described for the quantitative detn. of very small amts. of barbiturates in tissues and blood. Complete elimination of contaminants is not attempted. The difficulty due to the simultaneous extraction of barbiturate and impurities absorbing in the same region of the u-v. is overcome by the use of differential spectrophotometry, depending on extinction-pH relationships.