Identification of differentially expressed genes induced in pancreatic islet neogenesis
- 15 January 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 378 (3) , 219-223
- https://doi.org/10.1016/0014-5793(95)01457-8
Abstract
Cellophane wrapping of the hamster pancreas induces islet neogenesis. We have used the mRNA differential display technique to select for genes expressed during islet neogenesis but not in control pancreata. Ten candidate clones have been identified. Upon sequencing, 6 clones showed a high degree of homology to known genes, 1 showed some, and 3 showed no homology to genes of known sequence. Thus, mRNA differential display is a useful technique to identify genes induced during islet neogenesis, and in combination with screening hamster pancreatic cDNA libraries for full length clones, will enhance the likelihood of capturing the participants in this process.Keywords
This publication has 16 references indexed in Scilit:
- Expression of Reg gene in the Syrian golden hamster pancreatic islet regeneration modelDiabetologia, 1995
- Neutrophils and monocytes express high levels of PU.1 (Spi-1) but not Spi-BBlood, 1995
- Increased Ubiquitin Expression Suppresses the Cell Cycle Defect Associated with the Yeast Ubiquitin Conjugating Enzyme, CDC34 (UBC3)Published by Elsevier ,1995
- Loss of glucose-induced insulin secretion and GLUT2 expression in transplanted β-cellsDiabetes, 1995
- Identification of differentially expressed mRNA species by an improved disply technique (DDRT-PCR)Nucleic Acids Research, 1993
- Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimizationNucleic Acids Research, 1993
- Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain ReactionScience, 1992
- Expression of reg protein in rat regenerating islets and its co-localization with insulin in the Beta cell secretory granulesDiabetologia, 1990
- Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis.Journal of Clinical Investigation, 1989
- Isolation, characterization, and distribution of an unusual pancreatic human secretory protein.Journal of Clinical Investigation, 1985