Detection of RFLPs in Perennial Ryegrass, Using Heterologous Probes from Tall Fescue

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Abstract
Perennial ryegrass (Lolium perenne L.) and tall fescue (Festuca arundinacea Schreb.) are closely related species and both are widely used for turfgrass and forage. This study was conducted to determine if heterologous genomic clones from tall rescue could be used to detect restriction fragment length polymorphisms (RFLPs) in Lolium. Thirty‐nine randomly chosen clones from the PstI‐genomic library of hexaploid tall rescue were probed to EcoRI and HindIII digested genomic DNA from five perennial ryegrass genotypes, each from a different cultivar. Two‐thirds of the heterologous probes from tall rescue cross‐hybridized to Lolium DNA and detected high levels of polymorphism (69%). There were no significant relationships between polymorphisms and clone size or hybridizing fragment size. It was estimated that an average of 41.2% of the loci examined were homozygous, ranging from 21 to 58% among the five perennial ryegrass genotypes evaluated. Tall fescue clones used in this study exhibited the same cross‐hybridization behavior with Lolium as was observed with the diploid meadow fescue (F. pratensis Huds.) in a previous study, suggesting that the Lolium genome is closely related to that of F. pratensis. The tall rescue DNA clones characterized in this study will be useful in Lolium genetic and breeding studies.

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