Development of a highly sensitive fluorescence reaction detection system for liquid chromatographic analysis of reducing carbohydrates
- 1 January 1993
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 118 (7) , 773-776
- https://doi.org/10.1039/an9931800773
Abstract
A highly sensitive post-column detection system for reducing carbohydrates in liquid chromatography was developed using guanidine as the reagent. The limit of determination for pentose, 6-deoxyhexose and reducing saccharides was 5 pmol and that for hexose, N-acetylglucosamine and N-acetylneuraminic acid was 10 pmol. The calibration graphs were linear up to 1 nmol.Keywords
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