Effect of Phytolectins on Isolated Protoplasts from Plants
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 43 (1) , 33-44
- https://doi.org/10.1093/oxfordjournals.aob.a085608
Abstract
The effects of three phytolectins on isolated plant protoplasts have been examined. It was found that concanavalin A, phytohemagglutinin and wheat germ agglutinin were all capable of agglutinating protoplasts isolated from a variety of plant species. The incorporation of 3H-uridine, 3H-thymidine and 14C-leucine into RNA, DNA and protein respectively was also stimulated by the three lectins in isolated protoplasts. Treatment with α-methyl-D-mannoside abolished both the agglutinating and incorporation enhancing effects of concanavalin A (Con A) on barley protoplasts. Protoplasts with a regenerated cell wall also failed to respond to lectin treatment. These results suggest that lectin-binding to the protoplast membrane surface is required before either agglutination or metabolic stimulation will result.Keywords
This publication has 4 references indexed in Scilit:
- A Large-scale Isolation Procedure for Cereal Mesophyll ProtoplastsAnnals of Botany, 1979
- Studies on the Formation of Roots and Shoots in Wheat Callus CulturesAnnals of Botany, 1977
- Labelling of concanavalin A sites on the plasma membrane of soybean protoplastsProtoplasma, 1976
- Ultrastructural studies of the binding of concanavalin A to the plasmalemma of higher plant protoplastsPlanta, 1976