PLATELET MEMBRANE-GLYCOPROTEINS IN NORMAL DOGS AND DOGS WITH HEMOSTATIC DEFECTS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 93  (4) , 607-613
Abstract
Glycoproteins solubilized from membrane-enriched fractions of platelets from dogs with TT [thrombasthenic thrombopathia] or VWD [von Villebrand''s disease] and from normal controls were compared by SDS-PAGE [sodium dodecyl dodecyl sulfate-polyacrylamide gel electrophoresis]. The results were similar to those for the analogous human disorders. Normal and VWD canine platelet MP [membrane pellet] contained 3 major glycoproteins as well as 4 smaller MW proteins that stained for carbohydrate with the PAS [periodic acid-Schiff] reagent. MP from dogs with TT had a higher concentration of GP [glycoprotein with type only] I, a varied expression of GP II and a lower concentration of GP III. The findings re-emphasized the value of comparative studies of these models of human hemostatic defects.