HUMAN-LYMPHOCYTE MARKERS DEFINED BY ANTIBODIES DERIVED FROM SOMATIC-CELL HYBRIDS .2. A HYBRIDOMA SECRETING ANTIBODY AGAINST AN ANTIGEN EXPRESSED BY HUMAN LYMPHOCYTES-B AND LYMPHOCYTES-NULL

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (3) , 593-601
Abstract
A hybridoma (FMC4) was derived which secretes antibody showing selective reaction with human B [bone marrow-derived] lymphocytes, monocytes and some null lymphocytes. Few T [thymus-derived] lymphocytes in normal blood are stained, although stimulation of lymphocytes with PHA [phytohemagglutinin] leads to an increase in the proportion of cells reacting with the hybridoma antibody. The antibody reacts with B and null lymphoblastoid cell lines but not with T cell lines. B chronic lymphocytic leukemia (CLL) cells but not T-CLL are stained and null-type acute lymphoblastic leukemia (ALL) cells but not T-type ALL react. Normal blood myeloid cells do not react with FMC4 supernatant while some myeloid leukemias do. The expression of the antigen reacting with FMC4 supernatant suggests that FMC4 may secrete an antibody against the human equivalent of the Ia antigen.