Valence and Temporal Change in Affinity of Purified 7S and 18S Nurse Shark Anti-2,4 Dinitrophenyl Antibodies
Open Access
- 1 October 1972
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 109 (4) , 665-673
- https://doi.org/10.4049/jimmunol.109.4.665
Abstract
Purified shark 7S and 18S anti-2,4 dinitrophenyl (DNP) antibodies were purified by immunoadsorption. Comparative equilibrium dialysis studies with the 3H-ε-DNP-l-lysine ligand over a 20-month period indicated that the 7S antibody showed a 100-fold increase in the average intrinsic association constant. The binding affinity of the 18S molecule did not change significantly during the same period. Valences of 1.4 and 8 were measured for the 7S and 18S molecules respectively, purified from the 30-month bleedings.Keywords
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