Genetic analysis of adenovirus type 2. VIII. Physical locations of temperature-sensitive mutations

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Abstract
Temperature-sensitive mutations of human adenoviruses can be physically located on the viral genome by determining the DNA structures of recombinants formed in genetic crosses between different members of the same subgroup. The DNA structures of many interserotypic recombinants formed in crosses between temperature-sensitive (ts) mutants of adenovirus type 2 and adenovirus type 5 were analyzed with the restriction endonucleases BamHI, EcoRI, HindIII and Sma I. In this way, the physical coordinates of adenovirus type 2 (Ad2)ts1, Ad2ts3, Ad2ts4 and Ad2ts48 were mapped, and the mapping of Ad5ts1 was refined.