A NEW ASSAY-METHOD FOR LIPID PEROXIDES USING A METHYLENE-BLUE DERIVATIVE

  • 1 January 1985
    • journal article
    • research article
    • Vol. 10  (2) , 205-211
Abstract
To determine the absolute amount of lipid hydroperoxides in biological materials, a simple and sensitive colorimetric method was newly developed, based on the reaction of lipid hydroperoxides with a leucomethylene blue derivative in the presence of Hb. The amount of methylene blue formed was measured by it absorbance at 666 nm to calculate the amount of lipid hydroperoxides using cumene hydroperoxide as external standard. By this method, lipid hydroperoxide concentrations of < 7.5 nmol/tube were accurately determined.

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