Transformation of Penicillium chysogenum with selection for increased resistance to benomyl
- 15 July 1989
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 60 (2) , 165-168
- https://doi.org/10.1016/0378-1097(89)90501-6
Abstract
Incubation of protoplasts of Penicillium chrysogenum with the plasmid pBT-3 permitted selection of transformants on the basis of increased resistance to the anti-fungal agent benomyl. Transformants were obtained at a frequency of 1–2 per µg of DNA. Southern analysis revealed that transformation had occured by integration of vector sequences into the host genome.Keywords
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