Enhancement of Canine Isohemagglutinin A by a Heat Labile Serum Factor, Probably Complement.

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Abstract
Data are presented to clarify the nature of enhancement of the agglutinative capacity of the canine erythrocyte-isonatibody A by a heat-labile component of fresh dog serum. It was demonstrated by the antiglobulin technic (Coombs) that sensitization of A cells without maximum agglutination occurs if the serum complement or its component fractions are inactivated by binding divalent cation, separation of globulin fractions, heat, NH,3, zymosan or fixation with antigen-antibody complexes. Several heterologous sera were studied, of which only bovine serum was capable of enhancing the agglutinative capacity of canine anti-A. The possibility that this reaction represents a conglutination phenomenon is briefly discussed.