Action of Aldosterone on Citrate Synthase in Cultured Renal Collecting Duct Cells

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Abstract
Cultured renal collecting duct cells from neonatal rabbit kidney were used to examine the influence of aldosterone on enyzmatic activity of citrate synthase during increase in Na+ transport. Control epithelia showed citrate synthase activity of 71 ± 3 mU/mg protein (n=28), while after aldosterone treatment citrate synthase activity was significantly increased to 79 ± 6 mU/mgat 1 h(n=5), to 88 ± 6 mU/mg at 2 h(n=6) and to 93 ± 8 mU/mg protein at 3 h (n=5). Citrate synthase activity subsequently decreased to basal values. Spironolactone fully blocked the aldosterone-induced increase in citrate synthase activity. The time course of enzyme stimulation after aldosterone administration indicates that the hormone activates citrate synthase during the physiological early response phase.

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