Improved Techniques for Assessment of Plasma Lipoprotein Patterns. I. Precipitation in Gels after Electrophoresis with Polyanionic Compounds

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Abstract
A method is described for making lipoprotein fractions visible by polyanion precipitation in situ in the gels after electrophoresis. With the new technique the pattern and its interpretation in the differential diagnosis of all primary forms of hyperlipoproteinemia is the same as for the other lipid-staining procedures. The new technique is simpler to perform, reliable, and provides the required information 60 min after electrophoresis. It also allows a very fast determination of the abnormal lipoprotein (LP-X) that characterizes cholestasis, without use of a specific antiserum.