Viability of Cell Cultures Following Extended Preservation in Liquid Nitrogen

Publisher Website
Google Scholar
Abstract
Twenty-two frozen batches of 16 different cell lines certified by the Cell Culture Collection Committee were successfully stored in liquid nitrogen over an interval of 2.5 to 4.5 years. Confluent cell growth obtained in milk dilution bottles and roller .tubes prepared from thawed aimpules of frozen cells clearly demonstrated the high viability of these cell lines. The studies indicated that cells harvested and frozen under optimal conditions retained high viability during prolonged storage in liquid nitrogen, but that damaged cells as shown by initial low viability may be expected to show more variable results.