Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae

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Abstract
A rapid carbohydrate utilization procedure for the confirmation of N. gonorrhoeae and identification of other Neisseria spp. was developed. This method utilizes preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 .times. 50 mm) and micro-amounts of media (0.05 ml) make this method not only rapid, but also low in cost.