Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella
Open Access
- 1 May 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (5) , 2261-2263
- https://doi.org/10.1128/jcm.42.5.2261-2263.2004
Abstract
A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.Keywords
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