End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads
Open Access
- 15 November 2000
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 28 (22) , 98e-98
- https://doi.org/10.1093/nar/28.22.e98
Abstract
A novel approach for light-dependent covalent immobilisation of synthetic DNA oligomers to amino-coated paramagnetic beads is described. A hetero-bifunctional photo-reactive cross-linking chemical, 4-nitrophenyl 3-diazopyruvate, is applied to attach 5′ amino-modified DNA to both silica and polystyrene paramagnetic beads. The coupling yields are comparable with similar methods in which no photo-reactive chemicals are used. The immobilised DNA on the polystyrene and silica beads was used efficiently in hybridisation experiments. An extension of this approach to light-directed immobilisation of specific DNA to beads, located at different positions in micro-flow reactors, opens up a range of integrated applications to complex diagnostics, evolutionary biotechnology and novel areas such as DNA computing.Keywords
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