SUMMARY 1. Cortisone acetate suspension, administered subcutaneously or orally to rats, caused no changes in adrenal ascorbic acid concentration. Intraperitoneal injections of cortisone acetate suspension caused profound adrenal ascorbic acid depletion, which was apparently due to a non-specific effect caused by the intraperitoneal administration of suspended matter. 2. Pretreatment of rats with large doses of cortisone acetate diminished, but did not prevent completely, the adrenal ascorbic acid depletion normally caused by unilateral adrenalectomy.