THE QUANTITATIVE REDUCTION OF 2,3,5-TRIPHENYL TETRAZOLIUM CHLORIDE BY SKIN IN VITRO

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Abstract
The epidermis of human and guinea pig skin reduced this tetrazolium salt in vitro under anerobic incubation with or without added succinate substrate. The formazan is extracted in methyl cellosolve and is measured colori-metrically. It is stable for 48 hours. The method is quite reproducible for 10-20 mg (wet weight) and can be used for the comparison of the effect of drugs, heat and various methods of skin banking. Loss of tetrazolium reduction as a result of heat and certain enzyme inhibitors is accompanied by parallel loss of O2 consumption.