Glycoprotein nature of α2‐adrenergic receptors labeled with p‐azido[3H] clonidine in calf retina membranes
- 18 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (2) , 480-484
- https://doi.org/10.1016/0014-5793(88)80142-x
Abstract
α2,-Adrenergic receptors in calf retina membranes can be specifically labeled with the tritiated agonist p-azido[3H]clonidine. Saturation binding in the dark occurs with high affinity (1.3 ± 0.3 nM) to a single class of sites (1122 ± 67 fmol/mg protein). Irradiation of the membrane-bound radioligand results in the labeling of a peptide band with an apparent size of 65 kDa and a characteristic pharmacological profile for an α2-adrenergic receptor. The carbohydrate moieties of the α2-receptor are characterized by lectin affinity chromatography and glycosidase treatment. The Nonidet P-40-solubilized, p-azido[3H]clonidine-labeled receptors are completely retained by Con A- as well as WGA-Sepharose columns. Neuraminidase, α-mannosidase and TFMS do not affect the electrophoretic mobility of the receptor on SDS-PAGE whereas endoglycosidase F reduces the apparent size to 45 kDa.Keywords
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