Immobilization, stability and esterification studies of a lipase from a Bacillus sp.
- 1 August 2002
- journal article
- research article
- Published by Wiley in Biotechnology and Applied Biochemistry
- Vol. 36 (1) , 7-12
- https://doi.org/10.1042/ba20010070
Abstract
In the present paper a comparative account of the immobilization of a Bacillus lipase on different solid supports with different surface properties and their thermostability is presented. Immobilization enhanced the thermostability of lipase. At higher temperatures, lipase immobilized and cross-linked on a hydrophobic surface showed the maximum thermostability. The optimum temperature for immobilized lipase was 9 degrees C higher than for the free enzyme, while the pH optima were the same. The half-life of soluble lipase at 50 degrees C was calculated to be 4.5 h, while immobilized lipase did not lose any activity even after 8 h. The temperature stability (for 1 h) of immobilized enzyme was enhanced from 50 to 60 degrees C in comparison with non-immobilized enzyme. Applications of immobilized lipase for esterification are also presented.Keywords
This publication has 18 references indexed in Scilit:
- Immobilization of Pseudomonas cepacia lipase in a phyllosilicate sol–gel matrix: effectiveness as a biocatalystBiotechnology and Applied Biochemistry, 2000
- Enzymatic esterification of ethanol by an immobilised Rhizomucor miehei lipase in a perforated rotating disc bioreactorEnzyme and Microbial Technology, 2000
- Lipase-catalyzed synthesis of tricaprylin in a medium solely composed of substrates. Water production and eliminationEnzyme and Microbial Technology, 1997
- Influence of the organic solvents on the activity in water and the conformation of Candida rugosa lipase: Description of a lipase-activating pretreatmentEnzyme and Microbial Technology, 1996
- An investigation of crude lipases for hydrolysis, esterification, and transesterificationEnzyme and Microbial Technology, 1996
- Bioreactors with immobilized lipases: State of the artEnzyme and Microbial Technology, 1996
- Lipase activity of thermophilic bacteria from icelandic hot springsBiotechnology Letters, 1993
- Continuous hydrolysis of olive oil by immobilized lipase in organic solventBiotechnology & Bioengineering, 1992
- Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: Application in lipase-catalysed reactionsEnzyme and Microbial Technology, 1992
- A serine protease triad forms the catalytic centre of a triacylglycerol lipaseNature, 1990