Enzymatic activity and immunoreactivity of extracellular phospholipase A2 in inflammatory synovial fluids
- 1 October 1992
- journal article
- research article
- Published by Springer Nature in Inflammation
- Vol. 16 (5) , 451-457
- https://doi.org/10.1007/bf00918971
Abstract
Synovial fluid PLA2 concentration was measured by an ELISA technique using monoclonal antibodies raised against human recombinant “synovial-type” group II phospholipase A2. This ELISA was specific for synovial-type PLA2 and did not detect pancreatic (group I) PLA2. In all synovial fluids examined, including rheumatoid, osteoarthritic, psoriatic, and gouty fluids, synovial fluid PLA2 enzyme activity significantly correlated with PLA2 immunoreactivity (P2 activity by either putative PLA2 activating or inhibitory proteins.Keywords
This publication has 15 references indexed in Scilit:
- MEASUREMENT OF HUMAN PHOSPHOLIPASE A IN ARTHRITIS PLASMA USING A NEWLY DEVELOPED SANDWICH ELISARheumatology, 1992
- Circulating phospholipase A2 activity associated with sepsis and septic shock is indistinguishable from that associated with rheumatoid arthritisInflammation, 1991
- Acute Lung Injury Induced by Phospholipase A2: Structural and Functional ChangesAmerican Review of Respiratory Disease, 1991
- Compartmental heterogeneity of soluble phospholipases AInflammation, 1990
- Lipocortin and the mechanism of action of the glucocorticoidsBritish Journal of Pharmacology, 1988
- Modulation of phospholipase A2 activity in human synovial fluid by cationsInflammation, 1987
- Pancreatic Phospholipase A2: Isolation of the Human Gene and cDNAs from Porcine Pancreas and Human LungDNA, 1986
- Influence of plasma proteins on activity of proinflammatory enzyme phospholipase A2Inflammation, 1986
- Inflammatory Effect of Intradermal Administration of Soluble Phospholipase A2 in RabbitsJournal of Investigative Dermatology, 1986
- Characterization of extracellular phospholipase A2 in rheumatoid synovial fluidLife Sciences, 1985