INHIBITION OF GUINEA-PIG RENAL [Na + K]-ATPase BY NORMOTENSIVE HUMAN PLASMA: EFFECTS OF A HIGH SODIUM DIET
- 1 June 1983
- journal article
- Published by Wiley in Clinical and Experimental Pharmacology and Physiology
- Vol. 10 (3) , 261-264
- https://doi.org/10.1111/j.1440-1681.1983.tb00193.x
Abstract
The effect of plasma taken from normotensive humans, while on a low and high sodium diet, on [Na + K]-ATPase and 3H-ouabain binding was measured in tubules from guinea-pig kidneys. Plasma from the high sodium, compared to the low sodium, diet period: (a) inhibited [Na + K]-ATPase activity; (b) decreased 3H-ouabain affinity for binding sites; (c) increased the number of available 3H-ouabain binding sites; (d) decreased [Na + K]-ATPase turnover (activity/3H-ouabain binding sites). The inhibition of [Na + K]-ATPase suggests an increase in a (possible) natriuretic factor. The decreased affinity of 3H-ouabain binding suggests an endogenous ouabainoid, which may be the natriuretic factorKeywords
This publication has 8 references indexed in Scilit:
- Sodium Transport during the Natriuresis of Volume Expansion; a Study Using Peripheral Blood LeucocytesClinical Science, 1982
- Involvement of endogenous digitalis-like substance in genesis of deoxycorticosterone-salt hypertensionLife Sciences, 1982
- Effect of Acute and Chronic Salt Loading on Erythrocyte 22Na Efflux in Males with Essential HypertensionClinical Science, 1981
- EFFECT OF SODIUM INTAKE ON ABILITY OF HUMAN PLASMA TO INHIBIT RENAL Na+-K+-ADENOSINE TRIPHOSPHATASE IN VITROThe Lancet, 1981
- Endogenous digitalis-like substance in plasma of volume-expanded dogsNature, 1980
- A rapid sensitive method for determining phospholipid phosphorus involving digestion with magnesium nitrateLipids, 1979
- The modification of (Na+ + K+) ATPase sensitivity to ouabain after extraction of membrane constituents distinct from the enzymeBiochemical and Biophysical Research Communications, 1976
- Rates of dissociation of enzyme-ouabain complexes and K0.5 values in (Na+ + K+) adenosine triphosphatase from different speciesBiochemical Pharmacology, 1972