Urinary Excretion of32P and Desoxyribose in Rats Following Irradiation or Administration of a Single Dose of Methionine Sulphoximine

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Abstract
The urinary excretion of 32P was investigated in rats following the intraperitoneal administration of a single dose of 50 mg and 150 mg L-methionine sulphoximine, and compared with the effect of x-radiation, the administration of 2,4-dinitrophenol, and scalding of 50 per cent of the body surface. After the administration of a single dose of methionine sulphoximine, the excretion of 32P in the urine rises, depending on the amount administered. The effect of methionine sulphoximine and radiation on the urinary excretion of 32P is similar. A similar effect is observed after administration of 2,4-dinitrophenol, but scalding has the reverse effect, i.e. the excretion of 32P is reduced as compared with controls. After the administration of methionine sulphoximine, as after irradiation there is an increased excretion of Dische-positive substances, i.e. of urinary desoxyribose in rats. The increased urinary excretion of 32P and desoxyribose in rats may be due to a disorder in the mechanism of oxidative phosphorylation.