High-Titer Adeno-Associated Viral Vectors from a Rep/Cap Cell Line and Hybrid Shuttle Virus

Publisher Website
Google Scholar
Abstract
Adeno-associated virus (AAV) is a potential vector for in vivo gene therapy. A critical analysis of its utility has been hampered by methods of production that are inefficient, difficult to scale up, and that often generate substantial quantities of replication-competent AAV. We describe a novel method for producing AAV that addresses these problems. A cell line, called B50, was created by stably transfecting into HeLa cells a rep/cap-containing plasmid utilizing endogenous AAV promoters. Production of AAV occurs in a two-step process. B50 is infected with an adenovirus defective in E2b, to induce Rep and Cap expression and provide helper functions, followed by a hybrid virus in which the AAV vector is cloned in the E1 region of a replication-defective adenovirus. This results in a 100-fold amplification and rescue of the AAV genome, leading to a high yield of recombinant AAV that is free of replication-competent AAV. Intramuscular injection of vector encoding erythropoietin into skeletal muscle of mice resulted in supraphysiologic levels of hormone in serum that was sustained and caused polycythemia. This method of AAV production should be useful in scaling up for studies in large animals, including humans. Adeno-associated virus (AAV) holds tremendous promise for achieving stable transgene expression in the treatment of chronic diseases. Applications of this vector system to large animal models and humans will require improved methods of production. We describe a method of AAV production based on infection of a cell line expressing Rep and Cap with a hybrid virus in which AAV proviral vector sequence has been inserted into the E1 region of a recombinant adenovirus. The resulting method is easily scaled up and yields high quantities of recombinant vector without producing detectable replication-competent AAV.