Differential expression of α‐synuclein isoforms in dementia with Lewy bodies
- 9 July 2004
- journal article
- Published by Wiley in Neuropathology and Applied Neurobiology
- Vol. 30 (6) , 601-607
- https://doi.org/10.1111/j.1365-2990.2004.00572.x
Abstract
Dementia with Lewy bodies (DLB) is characterized by the widespread presence of Lewy bodies (LBs) in the brain. α‐Synuclein, the main component of LBs, is expressed as two main isoforms (112 and 140), but little is known about their differential expression in the brain. We compared α‐synuclein 112 and α‐synuclein 140 expression levels in the prefrontal cortices of six DLB patients, eight Alzheimer disease (AD) patients, and six control subjects. Relative α‐synuclein 112 and α‐synuclein 140 expression levels were determined by real‐time polymerase chain reaction with competimer technology using a LightCycler System. Whereas total α‐synuclein levels were just marginally elevated in DLB in comparison with the other groups, α‐synuclein 112 was seen to be markedly increased in DLB compared with AD cases and controls. In contrast, α‐synuclein 140 levels were significantly diminished in both neurodegenerative disorders in comparison with controls. These results show differential overexpression of α‐synuclein 112 in DLB, a finding that could be of importance in DLB pathogenesis.Keywords
This publication has 29 references indexed in Scilit:
- Conformational properties of α-synuclein in its free and lipid-associated states 1 1Edited by P. E. WrightJournal of Molecular Biology, 2001
- α-Synuclein Membrane Interactions and Lipid SpecificityJournal of Biological Chemistry, 2000
- Dopaminergic Loss and Inclusion Body Formation in α-Synuclein Mice: Implications for Neurodegenerative DisordersScience, 2000
- Mice Lacking α-Synuclein Display Functional Deficits in the Nigrostriatal Dopamine SystemNeuron, 2000
- Binding of α-Synuclein to Brain Vesicles Is Abolished by Familial Parkinson's Disease MutationJournal of Biological Chemistry, 1998
- Stabilization of α-Synuclein Secondary Structure upon Binding to Synthetic MembranesJournal of Biological Chemistry, 1998
- AlaSOPro mutation in the gene encoding α-synuclein in Parkinson's diseaseNature Genetics, 1998
- Mutation in the α-Synuclein Gene Identified in Families with Parkinson's DiseaseScience, 1997
- NACP, A Protein Implicated in Alzheimer's Disease and Learning, Is Natively UnfoldedBiochemistry, 1996
- Tissue-Dependent Alternative Splicing of mRNA for NACP, the Precursor of Non-Aβ Component of Alzheimer′s Disease AmyloidBiochemical and Biophysical Research Communications, 1994