Construction of cloning, promoter-screening, and terminator-screening shuttle vectors for Bacillus subtilis and Streptococcus lactis

1 August 1985

journal article
research article
Published by American Society for Microbiology in Applied and Environmental Microbiology

Vol. 50 (2) , 540-542
https://doi.org/10.1128/aem.50.2.540-542.1985

Abstract

Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.

Keywords

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