Mechanism of Polymerization of the Salmonella O-Antigen: Utilization of Lipid-Linked Intermediates
- 1 October 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (2) , 951-958
- https://doi.org/10.1073/pnas.67.2.951
Abstract
Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.Keywords
This publication has 18 references indexed in Scilit:
- Structure and Biosynthesis of the Bacterial Cell WallAnnual Review of Biochemistry, 1969
- Enzymic synthesis of O-antigen in Salmonella typhimuriumBiochemistry, 1968
- The metabolic basis for masking of receptor-sites on E. coli K-12 for C21, a lipopolysaccharide core-specific phageArchives of Biochemistry and Biophysics, 1968
- Mechanism of ϵ15 conversion studied with bacteriophage mutantsJournal of Molecular Biology, 1967
- The direction of chain growth in Salmonella anatum O-antigen biosynthesisBiochemical and Biophysical Research Communications, 1967
- Restriction of nonglucosylated T-even bacteriophage: Properties of permissive mutants of Escherichia coli B and K12Virology, 1967
- Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesisArchives of Biochemistry and Biophysics, 1966
- Biosynthesis of cell wall polysaccharide in mutant strains of Salmonella. IV. Synthesis of S-specific side-chainBiochemical and Biophysical Research Communications, 1965
- Separation of sugar nucleotides, phosphoric esters and free sugars by paper chromatography with solvents containing borates of organic basesJournal of Chromatography A, 1965
- Studies on the Chemical Basis of the Phage Conversion of O-Antigens in the E-Group Salmonellae*Biochemistry, 1962