ATTACHMENT OF PARTICLE-BOUND IGG AND COMPLEMENT TO HUMAN-NEUTROPHILS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 58  (4) , 772-781
Abstract
The attachment of particle-bound IgG in a nonphagocytic system stimulates formation of a microfilament-rich, organelle-poor zone in the subjacent cytoplasm of human neutrophils. The attachment site is characterized by ruffling and invagination of the neutrophil membrane. IgG attachment and formation of the organelle-poor zone are inhibited by the microfilament inhibitor cytochalasin B, but not by inhibitors of microtubules, such as colchicine or vinca alkaloids. Attachment of particle-bound complement is not inhibited by cytochalasin B in doses known to disrupt actin filaments. There is not discernible change in the subjacent-cytoplasm of the neutrophil in response to complement; the membrane attachment site is smooth, without ruffling or invagination. IgG-mediated attachment to neutrophils and its sequel, peripheral cytoplasmic reorganization, are evidently mediated by cytochalasin-sensitive structures, possibly actin. Complement-mediated attachment to neutrophils is insensitive to high doses of cytochalasin, suggesting that actin integrity is not required.

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