Platelet‐activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles
- 18 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 259 (1) , 209-212
- https://doi.org/10.1016/0014-5793(89)81530-3
Abstract
Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.Keywords
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