Formation of linoleic acid in the rat colon

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Abstract
1. The amino acid incorporating activity of skeletal muscle ribosomes was studied in rats under various nutritional conditions using labelled amino acids. 2. Ribosomes were obtained from rats that were given a protein-free diet for 5 d followed by a high-protein diet containing casein, gelatin or wheat gluten for 16.5 h and from others that were given one of these protein-containing diets or one containing protein from polished rice for 6 d. 3. The level of isotope incorporation relative to RNA was somewhat higher when the protein source given for 16.5 h was a good-quality protein such as casein than with gelatin or wheat gluten, but fine discrimination between proteins was not considered to be feasible with this system. 4. In the rats that were given the protein-containing diets for 6 d the differences were more pronounced and the amino acid incorporating activity was correlated with the biological value of the protein.