DOUBLE ANTIBODY SOLID-PHASE ASSAY FOR DNA AUTOANTIBODIES FOR CLINICAL USE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 25  (2) , 191-198
Abstract
DNA autoantibodies [human] in serum will bind to antigen-coated polystyrene tubes and can be detected by radiolabeled [rabbit] anti-immunoglobulin. The method is quantitative, gives information on the antibody class, and is not readily subject to interference by factors such as the size of the DNA, minor contamination of double-stranded with single-stranded DNA or the presence of materials which can combine non-specifically with the antigen. Double-stranded DNA gave good discrimination between SLE [systemic lupus erythematosus] and rheumatoid arthritis, but with single-stranded preparations approximately 40% of the RA patients showed elevated antibody values. Individual results obtained with the 2 antigens were compared and correlated with the Farr test and ANA [anti-nuclear antibody] titers. A proportion of the SLE sera gave high background binding to tubes coated with gelatin.