Identification of Clostridium butyricum and Clostridium beijerinckii by Gas-Liquid Chromatography and Sugar Fermentation: Correlation with DNA Homologies and Electrophoretic Patterns

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Abstract
Butyricum group clostridial strains (65) wee studied by DNA-DNA hybridization, electrophoresis of cell proteins, GLC and fermentation of glycerol, inositol and ribose. The DNA-DNA hybridization results confirmed that strains of this group belong to 2 main species, C. butyricum and C. beijerinckii. Five strains did not hybridize with the reference strains of these 2 spp. Most strains could be identified by quantitative GLC combined with fermentation patterns. The other strains could be identified by their protein electrophoretic patterns.