Rapid yeast DNA staining method for flow cytometry
- 10 January 1985
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 25 (8) , 509-512
- https://doi.org/10.1002/jobm.3620250812
Abstract
The variation of the fluorescence intensity of olivomycin‐stained yeast cells as a function of the concentration of olivomycin, NaCl, and MgCl2 in the staining solution was studied. The best results were obtained when the staining solution contained 100 μg olivomycin/ml, 40 mM MgCl2, and 1 M NaCl. A staining time of 12 min was sufficient for proper staining.This publication has 13 references indexed in Scilit:
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